EPA Method 1602: Male-Specific (F+) and Somatic Coliphage in Water by Single Agar Layer (SAL) Procedure (April 2001) - 

EPA 821-R-01-029

Summary Information:

  • Quantitative (PFU / 100mL) Method.
  • Single Agar Overlay procedure (overnight incubation).
  • up to 500 mL Sample Volume. Collect 250mL per phage (MS and or Somatic).
  • Hold Time = 48 hours.  Raw Sewage = 24 hrs
  • Store and Ship at 2 to 8 C
  • Matrix Spike sample on 1st Sample, 21st sample, etc. per matrix.
  • Male Specific and/or Somatic Coliphage
  • For Ground Water and other waters
  • Validated for Ground Water Only
  • This method is not for Biosolids


EPA Method 1601/1602



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